DIATHEVA - Biomanufacturing of innovative diagnostics and reagents

Alexandrium spp. PCR detection Kit

Alexandrium spp. PCR detection Kit allows the detection of Alexandrium DNA using polymerase chain reaction (PCR). PCR primers specifically detect all the Alexandrium species. The kit contains reagents and enzymes for the specific amplification of a 135 bp region of the Alexandrium 5.8S rDNA. This PCR assay is able to detect down to one cell per reaction. To check for possible PCR inhibition, an internal control, giving an amplicon of 217 bp, is also supplied.

Kit contents:

- 2 x 1ml Alexandrium spp. Mix - containing primers, dNTPs, 10X reaction buffer, MgCl2 and an internal control plasmid DNA including specific primers.
- 1 x 0,5ml Alexandrium Positive Control - DNA containing A.catenella specific sequence
- 1 x 10 μl Hot-Rescue DNA Polymerase
- 1 x 1ml PCR-grade Water

Price:
€ 270,00

Info

SUGGESTED COMPANION PRODUCTS:

#MBK0007 Dinoflagellate DNA isolation Kit

 

Identification of Alexandrium spp. DNA by polymerase chain reaction (PCR).

Paralytic shellfish poisoning (PSP) is a syndrome predominantly caused by the consumption of contaminated shellfish. PSP toxins are produced by dinoflagellates such as Alexandrium spp. Coastal water monitoring programmed to detect the presence of toxic algae could provide an essential tool to access bloom formation and consequently potential shellfish contamination. Usually, monitoring involves accurate morphological identification and the enumeration of target phytoplankton species in the seawater samples by using standard microscopy procedures. These methods are time-consuming and require a remarkable taxonomic experience. PCR-based methods are actractive as diagnostic tools because they can rapidly detect as low as one cell of specific organism in mixed ecological scenarios. These methods have been successfully applied to seawater samples for the identification of several dinoflagellates, reducing the time for the standard monitoring procedures.

 

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