DIATHEVA - Biomanufacturing of innovative diagnostics and reagents

Grain Quantitative Kit (150 reactions)

Grain quantitative kit allows the quantification of Triticum aestivum in Triticum spp. by quantitative Real-Time PCR. 

Grain quantitative kit is based on DNA detection that is more stable than other components even when subjected to technological processes used in the food industry. The DNA can be amplified and detected with high sensitivity and specificity, constituting an excellent molecular marker of the presence of soft wheat in the product (.. more..)



#MBK0064 Grains DNA Extraction Kit





€ 490,00



Grain quantitative kit could be used to determine the relative DNA copy number ratio of T.aestivum in Triticum spp. by comparing the amplification of a specific gene of T. aestivum (soft grain) with normaliser gene common of all species of Triticum genus. The relative quantification is obtained by the use of two calibration curves, one for each target gene.

Pasta is normally made from 100% durum wheat (Triticum turgidum) due to its special features to obtain a good final product.


The production and sale of pasta is strictly regulated by the current Italian law: only a maximum of 3% soft wheat (Triticum aestivum) can be tolerated in dry pasta to account for cross contamination that may occur during growing, harvesting and handling practices.

Sensibility: Limit of detection (LOD): 0,2% of T. aestivum in Triticum spp. Lower limit of quantification (LLOQ): 1% of T. aestivum in Triticum spp. Upper limit of quantification (ULOQ): 15% T. aestivum in Triticum spp.

Specificity: The specificity of the system was verified using DNA extracts from different species of genus Triticum and specific cultivar of T. aestivum. The species that have been tested for the amplification of the normaliser gene are: spelt (Triticum turgidum dicoccum), kamut (Triticum turgidum polonicum) durum wheat (Triticum turgidum) and soft wheat (Triticum aestivum). The species that have been tested for selective amplification of the target gene of T. aestivum are: rice (Oryza sativa), barley (Hordeum vulgare), maize (Zea mays), millet (Panicum miliaceum), oat (Avena sativa), spelt (Triticum turgidum dicoccum), kamut (Triticum turgidum polonicum), durum wheat (Triticum turgidum).


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