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Photobacterium damselae PCR Detection Kit

Photobacterium Damselae PCR detection Kit allows the detection of DNA from P. damselae using Polymerase Chain Reaction (PCR). PCR primer combination specifically discriminates both subspecies piscicida and damselae. The kit contains reagents and enzymes for the specific amplification of two target sequences: a 448 bp region specific for the subsp. damselae and a 297 bp region present in the genome of all Photobacterium damselae subsp. piscicida. Few P. damselae subsp. damselae isolates, showing the 297 bp amplicon, can be identified by the presence of the 448 bp product. To reveal possible PCR inhibition, an internal control, giving an amplicon of 112 bp, is also supplied in the PCR mix.

Kit contents:

- 2 x 1250μl Photobacterium damselae Mix
- 1 x 15μl Hot-Rescue DNA Polymerase (5U/μl)
- 1 x 15μl Photobacterium damselae Positive Control
- 1 x 1000μl PCR-Grade Water

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