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Human Anti LLO IgG Immunoassay CE IVD (ListeriaTEST)

Enzyme-linked immunosorbent assay (ELISA) for the detection of anti-LLO IgG in human serum and plasma to diagnose listerial infection and to screen people for L.monocytogenes exposure.


Listeria monocytogenes is a facultative intracellular Gram-positive food-borne bacterium, increasingly recognized as being responsible for severe infections in both animals and humans. Ingestion of contaminated food causes an infection, named listeriosis, which affects especially immunocompromised patients, new-borns and pregnant women and is characterized by a variety of severe syndromes, such as encephalitis, meningoencephalitis, septicemia and abortion. Listeriolysin O (LLO), a major virulence factor produced by all pathogenic strains of L. monocytogenes, has been identified as a candidate antigen for a serological assay. Antibodies to LLO have been already detected in the serum of goats, sheep, lambs, cows and humans by western blot, dot blot or ELISA analysis. (...more...)


The Listeria ELISA kit does not show cross reactivity with interfering antibodies present usually in the sera of pregnant women.


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Price:
€ 575,00

Info

(...more...) Detection of anti-LLO antibodies in humans has been proved to be particularly useful for listeriosis diagnosis especially when bacteria cannot be isolated from clinical specimens, owing to the intermittent presence in blood or the inaccessible foci of bacterial replication.

Microtiter strips coated with LLO are incubated with collected samples. During this incubation step, anti-LLO antibodies bind to the antigen forming specific complexes. Antibody excess is removed by washing and the antigen-antibody complex in each well is detected by adding an anti-human IgG HRP-conjugated antibody. Detection is performed by incubating the strips with 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) as chromogen and reading the absorbance values with a microplate reader at 415nm.

The kits allows 96 reactions; duplicates for each sample or control are strongly recommended.

Limitations of the procedure

• No drugs have been investigated for assay interference.

• The assay may cross react with Clostridium perfringens.

• Any variation in specimen diluent, operator, pipetting technique, washing technique, incubation time or temperature can cause variation in binding.

 

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