DIATHEVA - Biomanufacturing of innovative diagnostics and reagents

Multipathogen enrichment medium 500 gr.

Multipathogen enrichment medium broth could be used for the enrichment of Salmonella spp., L.monocytogenes and E. coli O157 in a food sample, prior to isolation procedures.Salmonella spp, L.monocytogenes, and E. coli O157 are known as major concern food-borne pathogens because of their continued association with highly popular foods such as dairy products. Above all, these pathogens have very high prevalence and mortality rates and have been involved in several recent outbreaks (Lynch et al. 2006). The Multipathogen enrichment medium provides nutrients and stable pH conditions to improve the recovery of L. monocytogenes, Salmonella spp. and E.coli O157 cells.


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Multiphatogen enrichment medium broth was formulated to allow the simultaneous growth of the mentioned species. This suggests that this medium, coupled with a suitable detection system, can facilitate the rapid detection of multiple pathogens concurrently from a food product, thus reducing the overall cost and time of pathogen testing.

Detection of foodborne pathogens on a single-assay platform provides results on the presence of pathogens in a single experiment. Though the sensitivities of many modern detection methods have improved significantly, such as nucleic acid amplification (Settanni and Corsetti, 2007). An enrichment step is still necessary to increase the target pathogens concentration, since they are often present in very low numbers in food samples.Currently, there is no single selective enrichment medium that can support the growth of E. coli , L.monocytogenes and Salmonella spp. concurrently.



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