Intended use: For identification of DPYD IVS14 + 1G>A polymorphism by High Resolution Melt (HRM).
Description: DPYD gene (located in 1p22) encodes dihydropyrimidine dehydrogenase protein, a pyrimidine catabolic enzyme representing the initial and rate-limiting factor in the pathway of uracil and thymidine catabolism. It is also the most important enzyme for degradation of 5-FU. Genetic variants causing DPYD deficiency leads to decreased 5-FU catabolism resulting in a significant increase in the effective 5-FU dose in the body. Several variants have been found in DPYD gene and among them IVS14 + 1G>A polymorphism represents one of the most predictive factor for 5-fluorouracil toxicity in cancer treated patients.