This assay is based on microplate enzyme immunoassay technique. Microtiter strips coated with antigen (prelamin A protein) are incubated with an optimized multi-enzymatic activity reaction mix containing nuclear extract samples. During incubation step, the enzymes in the target samples transform Prelamin A in its mature form. Therefore, mature lamin A formation can be detected as difference between total lamin and prelamin A signal and unconverted prelamin A signal. Revelation step is performed incubating the strips with 2,2’-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) as chromogen and reading the absorbance values at 415nm by ELISA micro-well plate reader.