Description: Human scFv Anti CEA clone E8 was selected from human ETH-2 library (Neri et al.) using Carcino Embrionic tumor associate Antigen (CEA) which is expressed on several cancer types including high malignant melanoma, breast and colon carcinomas. ScFv Anti CEA clone E8 contains: a phosphorylation site for labelling with radioactive P-32; a FLAG-tag sequence for detecting with the anti-FLAG-tag M2 antibody; the (His)6 sequence; the recognition site for the endopeptidase enterokinase.
Source: Recombinant protein expression in E.coli.
Recommended dilutions:
• ELISA: starting from 4μg/ml to 0.8μg/ml
• Flow Cythometry: starting from 5μg/ml to 1μg/ml
• Immunofluorescence: starting from 5μg/ml to 1μg/ml
Optimal dilution must be determined individually for each application.
Purity: >90% estimated by analysis of Comassie blue stained SDS – PAGE Purified by metal affinity chromatography
Form: Liquid, stabilized with 10% glycerol and EDTA 1.5 mM.
Storage: shipped at +4°C.When stored at +4°C the antibody is stable for 12 months. For extended storage, the solution may be frozen at –20°C in working aliquots.