Description: Vif (virion infectivity factor) protein is absolutely essential for productive HIV-1 infection of lymphocytes and macrophages. Vif is not required for production of infectious particles in several human cell lines.The viral infectivity factor (vif) induces productive viral replication by inactivating the host cell anti-HIV factor APOBEC3G. APOBEC3G (A3G) and related cytidine deaminases of the APOBEC3 family of proteins are potent inhibitors of many retroviruses, including HIV-1.
Source: Mouse monoclonal IgG1, 6
Recommended dilutions: Western blot starting dilution 0.2:g/ml- 1:g/ml Immunofluorescence starting dilution 1:g/ml -2:g/ml
Note: When using any primary antibody or fluorescence-labelled secondary antibody for the first time, titrate out the antibody to determine the best dilution for the strongest specific signal with the least background. As control add into the single slip a secondary antibody only.
Purity: Monoclonal immunoglobulins affinity purified
Clonality: Monoclonal
Form: Ready to use prediluted in Na+ Citrate 0.1 M, 0.05 mM Tris and 0.05% v/v of glycerol. The antibody solution is at neutral pH.
Storage: Shipped at +4°C . When stored at +4°C the antibody is stable for 12 months. For extended storage, the solution may be frozen at –20°C in working aliquots.