Description: The HIV-1 regulatory protein Tat is considered an attractive target for the development of a multicomponent vaccine against HIV-1 infection. The protein is well conserved among different isolates and thus may be less susceptible to mutation leading to the production of escape virus variants. Tat is produced early after infection and it’s essential for virus replication and infectivity. Tat protein is also immunogenic and antibodies (Ab) against Tat have been correlated with delayed disease progression and may exert protective effects inhibiting HIV replication Moreover, Tat is efficiently taken up by monocite-derived dendritic cells, promoting their maturation. Finally, murine vaccination with a biologically active Tat protein has been shown to be safe and immunogenic.
Source: Rabbit
Recommended starting dilutions:
Western Blotting: 1:20.000; ELISA: 1:10.000l; Immunofluorescence: 1:500; Cytoflurimetric assay (Facs): 1:500
Starting dilutions can vary lot-to-lot. Consult the product information label in the package for lot specific values
Purity: purified on Protein A affinity chromatography.
Clonality: Polyclonal
Form: Ready to use pre-diluted in Na+ Citrate 0.1M, 0.05mM Tris and 0.05% v/v of glycerol. The antibody solution is at neutral pH.
Storage: Shipped at +4°C. When stored at +4°C the antibody is stable for 18 months. For extended storage, the solution may be frozen at –20°C in working aliquots.